LIMITATIONS OF CELL-BASED
SYSTEMS |
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ADVANTAGES USING CELL-FREE |
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- Proteins that are toxic to cell-based systems
- Improper protein folding
- Require specialized post-translational modification
- Limited by recombinant guidelines
- Chimeric protein expression
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- May improve biologic activity and therapeutic effect and ability
to screen novel proteins that are not possible to make
using coventional systems
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- Potential for viral and prion contaminants
- Other antigenic contaminants
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- Elimination of viral contamination and validation
Cell-Free E.Coli system reduces potential for allergic or immunogenicity-type reactions
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- Low Protein Production and Yield
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- Direct control of synthesis results in improved protein yields and activity
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