Fundamental Applied Biology (FAB), Inc.: Cell-Free Technology Overview

FAB’s Cell-Free technology is specifically designed to overcome the limitations of current cell-based synthesis protein platforms.

Cell-Free technology has advantages that differentiate it from current cell-based systems, and may improve efficacy, safety, and speed of production due to more natural protein folding with better biologic activity, higher purity, lower degradation, and higher yields.

Cell-Free technology allows the synthesis of one desired target protein without the need for living cellular organisms. This is accomplished by extracting the necessary catalytic machinery required for metabolism, transcription, translation, and proper folding from living cells. The lack of a cell wall or membrane barrier, and requirements to satisfy biochemical needs associated with growth and maintenance of a living cell, provides critical advantages over conventional recombinant DNA protein expression technology. Thus, energy and other resources can be more focused and efficiently utilized for processes

directed toward synthesis and folding of the target therapeutic.

We believe these attributes may result in improved therapeutic efficacy, as well as reduced allergic reactions, immunogenicity, and viral contamination.

Cell-Free Uses an Efficient Energy System to Produce Therapeutic Proteins

Previous Cell-Fee systems have used costly and inefficient direct phosphate bond donation energy systems. FAB’s proprietary Cell-Free technology uses highly efficient oxidative phosphorylation to produce a stable, plentiful, and lasting energy source to drive protein synthesis.

Improved Protein Folding Is Critical For Biologic Activity

Proper disulfide bond formation is critical to the system’s ability to synthesize and properly fold complex protein therapeutics. FAB’s Cell-Free technology is specifically engineered for disulfide bond formation through sulfhydryl redox potential stabilization, adjustment, and catalysis. This amazing flexibility allows Cell-Free to synthesize and properly fold many targets that are difficult to produce using conventional recombinant protein expression platforms.

Cell-Free Allows A Seamless Transition to R&D Production

FAB’s Cell-Free technology is fully scalable from lab bench to production scale volume in batch mode. From preliminary product synthesis in the lab, to development of an early-stage production process, and making preclinical material, Cell-Free technology conserves performance at any scale. This allows a smoother transition from process development to R&D production. At larger scales, Cell-Free is designed to work with conventional bacterial cell culture reactors making the facility construction easy and development of new reactor technology unnecessary.